Isolation and Identification of Non-Conjugated Linoleic Acid from Processed Panax ginseng Using LC-MS/MS and 1H-NMR

Black ginseng exhibits numerous pharmacological activities due to higher and more diverse ginsenosides than unprocessed white ginseng. The ginsenoside derivatives have been investigated in order determine their chemical structures activities. We found a peak which was increased 10-fold but unidentified the methanol extracts of black product. unknown tracked identified as linoleic acid rather derivative using liquid chromatography–tandem mass spectrometry (LC-MS/MS) nuclear magnetic resonance (NMR) spectroscopy. NMR analysis confirmed no presence conjugated acids. Ginsenoside profiles contents products were quantified LC-MS/MS. Linoleic content directly proportional number applied thermal cycles manufacturing process any ginsenosides.